Molecular Biologists/Biochemists

at  University of Alberta

Department Faculty of Engineering - Electrical & Computer Engineering Dept
Posted date November 19, 2021
Closing date Will remain open until filled.
Position Type Full Time

DESCRIPTION

Professor Jie Chen’s lab in the Department of Electrical and Computer Engineering, at the University of Alberta, is recruiting molecular biologists/biochemists with strong backgrounds in single-cell RNA sequencing (scRNA-Seq) and excellent communication/collaboration ability to conduct the single-cell microfluidic platform project with engineers and other team members from diverse backgrounds.

Qualified candidates must have:

• Basic theological knowledge on various high-throughput single-cell RNA sequencing methods, especially Drop-Seq, microwell platform, InDrop and 10x chromium. Know the similarities and differences among these methods.
• Hands-on experiences in sequencing library construction workflow (especially 10x or Drop-Seq method), which includes reverse transcription, cDNA tagmentation with Nextera XT, PCR amplification, cDNA purification by AMPure XP and cDNA library quality check with BioAnalyzer.
• Knowledge on genomics/transcriptomics data analysis is an asset, which includes any of the following:
• Sequencing data quality control and mapping of raw sequencing reads using spliced transcripts alignment tool (e.g.STAR aligner).
• Demultiplexing of sequencing data to generate the gene expression (count) matrix.
• Downstream data analysis and interpretation,including clustering (using the Seurat Package), Gene Set Enrichment analysis (GESA) to explore the effect of DEP force manipulation on gene expression and cell cycle analysis based on the expressed gene sequenced by scRNA-Seq, ect.

At the University of Alberta, we are committed to creating an inclusive and accessible hiring process for all candidates. If you require accommodations to participate in the interview process, please let us know at the time of booking your interview and we will make every effort to accommodate your needs.
All qualified candidates are encouraged to apply; however, Canadians and permanent residents will be given priority. If suitable Canadian citizens or permanent residents cannot be found, other individuals will be considered.
We thank all applicants for their interest; however, only those individuals selected for an interview will be contacted.
The University of Alberta is committed to an equitable, diverse, and inclusive workforce. We welcome applications from all qualified persons. We encourage women; First Nations, Métis and Inuit persons; members of visible minority groups; persons with disabilities; persons of any sexual orientation or gender identity and expression; and all those who may contribute to the further diversification of ideas and the University to apply

Please refer the Job description for details