Laboratory Assistant

at  UNIVERSITY OF CALIFORNIA IRVINE

Overview:
Founded in 1965, UC Irvine is a member of the prestigious Association of American Universities and is ranked among the nation’s top 10 public universities by U.S. News & World Report. The campus has produced five Nobel laureates and is known for its academic achievement, premier research, innovation and anteater mascot. Led by Chancellor Howard Gillman, UC Irvine has more than 36,000 students and offers 224 degree programs. It’s located in one of the world’s safest and most economically vibrant communities and is Orange County’s second-largest employer, contributing $7 billion annually to the local economy and $8 billion statewide.
To learn more about UC Irvine, visit www.uci.edu.
Today’s UC Irvine Stem Cell Research Center is a dynamic center encompassing a diverse array of interdisciplinary investigators that span the schools of Medicine, Biological Sciences, Pharmaceutical Sciences, Engineering, Arts and Law. Our state-of-the-art, LEED Platinum certified research and clinical building houses 23 of our 50 center faculty members.
Center faculty have obtained more than $170 million dollars in grant and foundation funds. Our research programs include an National Institutes of Health (NIH)-sponsored T32 training grant, state of the art shared equipment, research services programs and a seminar series.
Job duties include, but are not limited to:
Animal care and Histology:
Assists in animal laboratory research projects. Research projects may consist of: Assist with breeding colonies (setting up cages, tissue collection for genotyping, detailed record keeping), perform survival and terminal surgical procedures in rodents. Daily monitoring of animals for health. Assist with histological analyses including tissue sectioning staining and/or quantification using stereological analysis of CNS tissue samples using MicroBrightField StereoInvestigator, Volocity and Imaris image analysis software.
Cell and Molecular Biology:
Duties may include maintenance of multiple cell lines (mouse or human stem cells, cortical cells, etc.), cell culture assays, flow cytometry and immunocytochemistry, such as DNA/RNA/Protein isolation and electrophoresis, PCR, rtPCR, qPCR, Western Blot, Molecular Cloning, ELISA.
Cell culture and differentiation duties may include isolate neural stem cells by mouse brain microdissection, passage, maintain, expand and freeze human and mouse neural and pluripotent stem cells. Follow written protocols to perform neural induction from pluripotent cells. Follow supervisor’s instructions on animal husbandry.
Cellular Analysis may include cell analysis by immunocytochemistry, flow cytometry and FACS. May be required to follow written protocols for engineering/bioengineering cell analysis platforms, 3D biomaterials, and sectioning.
General Lab Support
Maintain lab databases for ordering and inventory. Follow written instructions to prepare stock solutions, cell culture media, and assist other researchers in the lab.
Responsibilities:
Under supervision of the PI, or other senior lab personnel, the incumbent may perform tasks including but not limited to: assisting with experiments, working as part of a team, and independent tasks; attending staff meetings; writing/updating/distributing lab protocols, SOPS, project plans/summaries/reports; training new personnel; preparing solutions, maintaining detailed paper and electronic records of study data; adhering to all experimental protocol and good laboratory practices, maintaining safety training and safe work procedures; lab maintenance including supply inventory, equipment maintenance, cleaning, organizing, and managing lab spaces and materials.
Additional tasks: Animal care and histology; maintenance of multiple cell lines (mouse or human stem cells, cortical cells, etc.), cell culture assays, flow cytometry and immunocytochemistry, such as DNA/RNA/Protein isolation and electrophoresis, PCR, rtPCR, qPCR, Western Blot, Molecular Cloning, ELISA. Cell culture and differentiation duties; Cellular Analysis may include cell analysis by immunocytochemistry, flow cytometry and FACS. May be required to follow written protocols for engineering/bioengineering cell analysis platforms, 3D biomaterials, and sectioning. and performing other duties as assigned.
Qualifications:

Please refer the Job description for details