Lentiviral Production Co-Op at Legend Biotech

Philadelphia, Pennsylvania, United States -

Full Time

Start Date

Immediate

Expiry Date

17 Feb, 26

Salary

0.0

Posted On

20 Nov, 25

Experience

0 year(s) or above

Remote Job

Yes

Telecommute

Yes

Sponsor Visa

Skills

Cell Culture, Virology, Bioprocessing, Laboratory Techniques, Pipetting, Buffer Preparation, Aseptic Handling, Chromatography Systems, Tangential Flow Filtration, Analytical Testing, Documentation Skills, Attention to Detail, Organizational Skills, Team Collaboration, Troubleshooting, Process Optimization

Industry

Biotechnology Research

Description

Legend Biotech is a global biotechnology company dedicated to treating, and one day curing, life-threatening diseases. Headquartered in Somerset, New Jersey, we are developing advanced cell therapies across a diverse array of technology platforms, including autologous and allogenic chimeric antigen receptor T-cell, T-cell receptor (TCR-T), and natural killer (NK) cell-based immunotherapy. From our three R&D sites around the world, we apply these innovative technologies to pursue the discovery of safe, efficacious and cutting-edge therapeutics for patients worldwide. Legend Biotech entered into a global collaboration agreement with Janssen, one of the pharmaceutical companies of Johnson & Johnson, to jointly develop and commercialize ciltacabtagene autolecuel (cilta-cel). Our strategic partnership is designed to combine the strengths and expertise of both companies to advance the promise of an immunotherapy in the treatment of multiple myeloma. Legend Biotech is seeking Lentiviral Production Co-Op as part of the R&D team based in Philadelphia, PA. Role Overview The Lentiviral Production Co-op will support upstream and downstream processes for lentiviral vector manufacturing, including cell culture, transfection, purification, and analytical testing in a BSL-2 environment. This hands-on role offers exposure to industry-standard techniques and equipment while collaborating with scientists and engineers to optimize workflows for gene therapy applications. Co-Op Duration: 6-months (Summer to Fall) with possible extension Key Responsibilities Assist in culturing suspension and adherent HEK293 cells Support lentiviral transfection and harvest process, including media preparation and monitoring cell growth and viability Perform buffer preparation, system setup, and cleaning following established SOPs. Operate AKTA chromatography systems for purification steps (ion-exchange and/or affinity) Perform Tangential flow filtration (TFF) for concentration and buffer exchange of lentiviral vectors Monitor process parameters and troubleshoot basic issues under supervision Assist in formulation, sterile filtration, and aliquoting of purified lentiviral products Perform lentiviral tittering using functional assays (TU/mL) and p24 ELISA for capsid quantification Conduct endotoxin testing to ensure product safety and compliance with regulatory standards Collect, analyze, and document process data to support optimization studies. Maintain accurate laboratory records and ensure compliance with safety and quality standards. Collaborate with scientists and engineers to troubleshoot and improve purification workflows. Requirements Currently enrolled in Master’s program in Biotechnology, Biochemical engineering, molecular biology, or related field. Basic understanding of cell culture, virology, or bioprocessing principles preferred. Familiarity with laboratory techniques such as pipetting, buffer preparation, and aseptic handling. Familiarity with chromatography systems (AKTA) and TFF is a plus. Comfortable working in a BSL-2 environment. Strong attention to detail, organizational skills, and ability to work independently and in a team environment. Excellent communication and documentation skills. What we offer: Hands-on experience in viral vector production processes such as upstream, downstream, and analytics. Opportunity to work in a collaborative and innovative environment. Exposure to industry-standard equipment and processes in gene therapy manufacturing. #Li-JR1 #Li-Onsite Benefits We are committed to creating a workplace where employees can thrive - both professionally and personally. To attract and retain top talent in a highly competitive industry, we offer a best-in-class benefits package that supports well-being, financial stability, and long-term career growth. Our offerings are designed to meet the diverse needs of our team members and their families, ensuring they feel valued and supported every step of the way. Highlights include medical, dental, and vision insurance as well as a 401(k)-retirement plan with company match that vest fully on day one. Equity and stock options are available to employees in eligible roles, we offer eight weeks of paid parental leave after just three months of employment, and a paid time off policy that includes 15 vacation days, 5 personal days, 5 sick days, 11 U.S. national holidays, and 3 floating holidays. Additional benefits include flexible spending and health savings accounts, life and AD&D insurance, short- and long-term disability coverage, legal assistance, and supplemental plans such as pet, critical illness, accident, and hospital indemnity insurance. We also provide commuter benefits, family planning and care resources, well-being initiatives, and peer-to-peer recognition programs - demonstrating our ongoing commitment to building a culture where our people feel empowered, supported, and inspired to do their best work. EEO Statement Legend Biotech is a proud equal opportunity/affirmative action employer committed to attracting, retaining, and maximizing the performance of a diverse and inclusive workforce. It is Legend’s policy to ensure equal employment opportunity without discrimination or harassment based on race, color, religion, sex (including pregnancy, childbirth, or related medical conditions), sexual orientation, gender identity or expression, age, disability, national origin, marital or domestic/civil partnership status, genetic information, citizenship status, uniformed service member or veteran status, or any other characteristic protected by applicable law. Employment is at-will and may be terminated at any time with or without cause or notice by the employee or the company. Legend may adjust base salary or other discretionary compensation at any time based on individual, team, performance, or market conditions. Legend Biotech maintains a drug-free workplace.

Responsibilities

The Lentiviral Production Co-op will support upstream and downstream processes for lentiviral vector manufacturing, including cell culture, transfection, purification, and analytical testing. This role involves collaborating with scientists and engineers to optimize workflows for gene therapy applications.