PhD scholarship in Probing the Physiological Limits of Microbial Acetate As at Danmarks Tekniske Universitet

Copenhagen, Capital, Denmark -

Full Time

Start Date

Immediate

Expiry Date

01 Oct, 26

Salary

0.0

Posted On

03 Jul, 26

Experience

0 year(s) or above

Remote Job

Yes

Telecommute

Yes

Sponsor Visa

Skills

Microbial Physiology, Intermediary Metabolism, Anaerobic Techniques, Microaerobic Techniques, GC-FID, LC-MS, 13C-labelling, Molecular Biology, Genetic Engineering, Adaptive Laboratory Evolution, Transcriptomics, Proteomics, Metabolomics, Metabolic Engineering, Substrate Quantification, High-throughput Cultivation

Industry

Research Services

Description

If you are curious about the fundamental physiology of microbial metabolism and excited by the prospect of pushing microorganisms to their biochemical limits, this is your opportunity. At DTU, you will investigate how microbial cells can be empowered to assimilate acetate more efficiently by supplying external reducing power — contributing to a deeper understanding of microbial physiology while helping unlock the potential of Power-to-X feedstocks for sustainable bioproduction. Responsibilities and qualifications Acetate is a promising CO₂-derived P2X feedstock, but its assimilation is energetically costly for microbial cells. In this project, you will investigate whether co-feeding reduced C1 compounds such as methanol can relieve these redox constraints and push the carbon-efficiency limits of acetate assimilation — using a genetically tractable model bacterium that naturally encodes the relevant metabolic pathways. Specifically, you will: * Establish and characterize stable acetate–C1 co-utilization phenotypes using adaptive laboratory evolution (ALE) and/or targeted metabolic engineering * Quantify carbon yields and biomass during co-utilization and validate stoichiometric predictions of carbon retention across different acetate assimilation pathways * Map physiological trade-offs and substrate partitioning across acetate:C1 ratios using high-throughput cultivation systems, ¹³C-labelling, and metabolite quantification * Investigate how the redox entry point of C1 oxidation controls carbon assimilation flux and retention efficiency * Apply transcriptomics, proteomics, and metabolomics to identify the regulatory and metabolic adaptations enabling efficient redox balancing between substrates of distinct reduction states * Disseminate results through peer-reviewed publications and presentations at international conferences You must have a two-year master's degree (120 ECTS points) or equivalent. The ideal candidate has: * A MSc in microbiology, biochemistry, microbial physiology, or a related field * A strong foundation in microbial physiology and intermediary metabolism * Hands-on experience with microbial cultivation, including anaerobic or microaerobic techniques * Experience with analytical methods for metabolite and substrate quantification (e.g. GC-FID, LC-MS, ¹³C-labelling) * Familiarity with molecular biology and basic genetic engineering in bacteria * Curiosity, scientific rigour, and genuine enthusiasm for fundamental physiological questions * A collaborative mindset and excellent communication skills in English Approval and Enrolment The scholarship for the PhD degree is subject to academic approval, and the candidate will be enrolled in one of the general degree programmes at DTU. For information about our enrolment requirements and the general planning of the PhD study programme, please see DTU's rules for the PhD education [https://www.dtu.dk/english/Education/PhD/Rules]. Assessment Candidates will be assessed on the basis of their academic background in microbial physiology and metabolism, their hands-on laboratory experience, and their motivation for fundamental physiological research. Shortlisted candidates will be contacted for a formal interview with the principal supervisor and the co-supervisor. We offer DTU is a leading technical university globally recognized for the excellence of its research, education, innovation and scientific advice. We offer a rewarding and challenging job in an international environment. We strive for academic excellence in an environment characterized by collegial respect and academic freedom tempered by responsibility. Salary and appointment terms The appointment will be based on the collective agreement with the Danish Confederation of Professional Associations. The allowance will be agreed upon with the relevant union. The period of employment is 3 years. Starting date is January 1st, 2027 (or according to mutual agreement). The position is a full-time position. You can read more about career paths at DTU here [http://www.dtu.dk/english/about/job-and-career/working-at-dtu/career-paths]. Further information Further information may be obtained from Enrico Orsi (enricoo@dtu.dk) or co-supervisor Ruud Weusthuis (ruud.weusthuis@wur.nl), Wageningen University and Research. You can read more about us at www.bright.dtu.dk [http://www.bright.dtu.dk] If you are applying from abroad, you may find useful information on working in Denmark and at DTU at DTU – Moving to Denmark [https://www.dtu.dk/english/about/job-and-career/moving-to-denmark]. Furthermore, you have the option of joining our monthly free seminar “PhD relocation to Denmark and startup “Zoom” seminar [https://www.dtu.dk/english/about/job-and-career/moving-to-denmark/before-arrival/phd-relocation-to-denmark]” for all questions regarding the practical matters of moving to Denmark and working as a PhD at DTU. Application procedure Your complete online application must be submitted no later than 3 August 2026 (23:59 Danish time). Applications must be submitted as one PDF file containing all materials to be given consideration. To apply, please open the link "Apply now", fill out the online application form, and attach all your materials in English in one PDF file. The file must include: * A letter motivating the application (cover letter) * Curriculum vitae * Grade transcripts and BSc/MSc diploma (in English) including official description of grading scale You may apply prior to obtaining your master's degree but cannot begin before having received it. Applications received after the deadline will not be considered. All interested candidates irrespective of age, gender, disability, race, religion or ethnic background are encouraged to apply. As DTU works with research in critical technology, which is subject to special rules for security and export control, open-source background checks may be conducted on qualified candidates for the position. BRIGHT – Biotechnology Research Institute for the Green Transition The Novo Nordisk Foundation Biotechnology Research Institute for the Green Transition (BRIGHT) is a flagship initiative hosted at DTU, one of the world's top 5 universities in biotechnology research and innovation. BRIGHT connects and amplifies biotechnology and bioproduction research, innovation, and education across the entire value chain — from bold ideas in the lab to real-world implementation in industry. Focused on three missions — sustainable materials, microbial foods, and microorganisms for net-zero agriculture — BRIGHT aims to accelerate the development of scalable biosolutions for Europe and beyond, guiding the way towards a more sustainable, circular, and competitive bioeconomy. DTU – For the benefit of society since 1829 DTU is one of Europe's leading elite technical universities. Through research and education at an international top level, we create solutions to the major societal challenges of our time and help secure Europe's global leadership in sustainable technological development. Since Hans Christian Ørsted founded DTU almost 200 years ago, our mission has remained the same: We develop and create value through the natural and technical sciences for the benefit of society. DTU has 13,800 students, 1,600 PhD students, and 6,500 employees. We work in an international environment and have an inclusive, stimulating, and informal work culture. DTU has campuses in all parts of Denmark and in Greenland and collaborates with the best universities around the world.

Responsibilities

Investigate how external reducing power and C1 compounds can improve the efficiency of microbial acetate assimilation. The role involves using adaptive laboratory evolution and multi-omics techniques to map physiological trade-offs and disseminate findings through publications.