On-Board Scientific is hiring a Protein Expression and Purification Scientist in South San Francsico
For immediate consideration please send your resume to resumes@onboardusa.com
Subject Line: Position Title and State you are located

OVERVIEW:

We are seeking a motivated Scientist to support the Protein Science team in the production of recombinant proteins, monoclonal antibodies, multispecific scaffolds, and novel protein modalities for early discovery research. This individual will play a key role in executing and developing mammalian cell culture and purification workflows.

Responsibilities:

• Maintain and propagate CHO cells in suspension culture to support ongoing projects.
• Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293).
• Express and purify a variety of proteins including monoclonal and bispecific antibodies, VHHs, Fabs, and Fc-Fusions using resin and magnetic-bead immunoprecipitation methods.
• Execute fed-batch experiments across multiple platforms including shake flasks (125 mL to 5 L), tube spins, and multi-well plates.
• Conduct analytical measurements including:
• Cell growth analysis
• Metabolite profiling
• HPLC-based titer analysis
• SDS-PAGE, UPLC, CESDS, NanoDrop, UV-Vis
• Maintain and troubleshoot mammalian cell culture systems.
• Accurately document all experimental data and processes using electronic lab notebooks (ELN).
• Prepare reports and effectively communicate results to internal teams.

Required Qualifications:

• Education:
• B.S. in Biochemistry, Cell/Molecular Biology, Bioprocess Engineering, or a related field with at least 3 years of experience in industry or academia
• OR M.S. degree with at least 1 year of relevant experience
• Technical Experience:
• Hands-on experience with mammalian cell culture and media preparation
• Proficient in aseptic techniques and use of single-use systems such as deep-well plates and shake flasks
• Demonstrated ability to perform both transient and stable transfections in CHO and HEK293 cells
• Foundational experience with protein purification and basic quality control

Preferred Qualifications:

• Experience with high-throughput and automated systems
• Experience in batch purification using a variety of scaffolds
• Prior industry experience is highly desirable

Software & Analytical Skills:

• Familiarity with biological data collection and instrumentation tools (e.g., Vi-Cell, metabolite analyzers)
• Proficiency in MS Office (Word, Excel, PowerPoint) and electronic lab notebooks (ELN)
• Experience with data/reagent management tools such as Dotmatics, Benchling, or Spotfire (preferred

Soft Skills & Work Expectations:

• Excellent communication, organizational, and interpersonal skills
• Ability to multi-task across concurrent protocols and maintain attention to detail
• Demonstrated problem-solving and troubleshooting skills in cell culture systems
• High level of flexibility and responsiveness to changing priorities
• Strong work ethic, sense of urgency, and ability to work independently
• Must be physically comfortable with extended periods of standing, walking, and working at biosafety cabinets

• Maintain and propagate CHO cells in suspension culture to support ongoing projects.
• Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293).
• Express and purify a variety of proteins including monoclonal and bispecific antibodies, VHHs, Fabs, and Fc-Fusions using resin and magnetic-bead immunoprecipitation methods.
• Execute fed-batch experiments across multiple platforms including shake flasks (125 mL to 5 L), tube spins, and multi-well plates.
• Conduct analytical measurements including:
• Cell growth analysis
• Metabolite profiling
• HPLC-based titer analysis
• SDS-PAGE, UPLC, CESDS, NanoDrop, UV-Vis
• Maintain and troubleshoot mammalian cell culture systems.
• Accurately document all experimental data and processes using electronic lab notebooks (ELN).
• Prepare reports and effectively communicate results to internal teams