POSITION SUMMARY/ DEPARTMENT SUMMARY:

The Kalish lab is a developmental neurobiology research group focused on how early life exposures, including during pregnancy, impact lifelong brain health and subsequent risk of neuropsychiatric disease. Our lab leverages advanced molecular and genomic approaches, including spatial transcriptomics, single cell sequencing, and human cell culture, to define molecular mechanisms underlying fetal and neonatal brain injury and neurodevelopmental disorders. We are seeking an experienced research assistant to perform human induced pluripotent stem cell culture, as well as neural progenitor and human microglia cell culture. The successful candidate will also perform immunocytochemistry and preparation of next generation sequencing libraries. Experience with mouse models is also possible. This is an ideal position for a candidate prior to graduate school, or can be a position for long-term scientific and career growth. Experience with neurobiology and neuroimmunology is preferred.

Key Responsibilities:

• Maintain human stem cell cultures and perform differentiation to neural progenitor cells and microglia.
• Maintain mouse colony and perform experiments in mouse models.
• Calculates, graphs and compiles data obtained, maintaining records and logs of work performed; perform statistical analyses.
• Prepares articles and papers on specialized techniques.
• Stays abreast in safety procedures, storage and disposal of bio hazardous, infectious, corrosive, radioactive and toxic substance, regulations in the lab to adhere to new Hospital and federal mandates.

MINIMUM QUALIFICATIONS

Education:

• Bachelor’s Degree in STEM.

Experience:

• 1 year of related work experience required, 2 years preferred.
• Human cell culture experience, iPS and/or organoid based culture experience is ideal.
• Experience with mouse models.
• Experience with immune profiling and flow cytometry.
• Analytical skills to resolve complex problems requiring the use of scientific, mathematical, or technical principles and in depth, experienced based knowledge.
• Ability to communicate effectively both orally and in writing including assigning and distributing work, coaching, training and enforcing policies.
• Strong attention to detail and independent problem-solving is a must.

Boston Children’s Hospital offers competitive compensation and unmatched benefits including flexible schedules, affordable health, vision and dental insurance, child care and student loan subsidies, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition and certain License and Certification Reimbursement, cell phone plan discounts and discounted rates on T-passes. Experience the benefits of passion and teamwork.

• Maintain human stem cell cultures and perform differentiation to neural progenitor cells and microglia.
• Maintain mouse colony and perform experiments in mouse models.
• Calculates, graphs and compiles data obtained, maintaining records and logs of work performed; perform statistical analyses.
• Prepares articles and papers on specialized techniques.
• Stays abreast in safety procedures, storage and disposal of bio hazardous, infectious, corrosive, radioactive and toxic substance, regulations in the lab to adhere to new Hospital and federal mandates