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MINIMUM EDUCATION & EXPERIENCE

• High School Diploma required.
• Associate’s degree in appropriate discipline (preferably biology).
• 7 years of relevant experience; or equivalent combination of education and experience required.3-4 years specialized experience in related field; Previous work experience in a research lab highly preferred.

KNOWLEDGE, SKILLS AND ABILITIES

• Mac, PC, and MS Office. Pipettes, gel apparatus, incubator shaker, centrifuges, -80 freezer, Barnstead Water Units, NuAire Bio-safety cabinet, dissecting microscopes, various surgical instruments, PCR machine, down draft tables, perfusion apparatus, CO2 euthanasia stations, ventilated rodent housing racks.
• OSHA safety requirements: scientific methods required
  The University of Rochester is committed to fostering, cultivating, and preserving an inclusive and welcoming culture to advance the University’s Mission to Learn, Discover, Heal, Create – and Make the World Ever Better. In support of our values and those of our society, the University is committed to not discriminating on the basis of age, color, disability, ethnicity, gender identity or expression, genetic information, marital status, military/veteran status, national origin, race, religion, creed, sex, sexual orientation, citizenship status, or any other characteristic protected by federal, state, or local law (Protected Characteristics). This commitment extends to non-discrimination in the administration of our policies, admissions, employment, access, and recruitment of candidates, for all persons consistent with our values and based on applicable law.

GENERAL PURPOSE

The Anderson lab in the Cardiovascular Research Institute is looking for a highly motivated Laboratory Technician to assist with animal and cell-based experiments and with maintaining a mouse colony in the general support of a molecular biology research laboratory. The individual will be expected to achieve the ability to function independently. Excellent organizational skills and the ability to multi-task are required. All necessary training will be provided, however experience in mouse handling, mammalian cell culture and transfection, and molecular biology techniques, such as Western blotting, PCR, qPCR, cloning, etc, are a plus. Under general guidance from the PI with latitude for exercising considerable independent judgment of handling lab techniques. This position has no official supervisory responsibility.

ESSENTIAL FUNCTIONS

• Perform molecular biology techniques and experiments in support of a research lab, ex. bacterial culture, mini- and maxi-prep of plasmid DNA, maintenance of mammalian cell culture lines, yeast culture, protein purification, etc.
• Animal colony management for mouse colonies. Animal husbandry to include breeding, weaning, pairing, tail snip, and inventory maintenance.
• Data entry into established database and spreadsheet for management of animal colonies; inventory control of animal numbers and UCAR accounts
• Perform tissue harvests from adult mice and staged embryos and process using experimental procedures related to gene expression analysis (Realtime PCR, western blot, immunohistochemistry, in situ hybridization. lacZ reporter activity, etc.).
• Independently and proactively perform general laboratory duties such as preparing various culture media (mammalian and bacterial), chemical solutions, reagents and re-stocking supplies for use by all lab members.
• Inventory lab materials and order supplies, and tracking spending.
• Write new laboratory formulae, procedures and protocols. Perform the technical training for new lab members including postdoctoral fellows in the lab: including animal handing and breeding, mouse genotyping,
• Western blotting, bacteria plasmid culture and DNA purification.
  Other duties as assigned