SRA Protein Expression at Nurix

Brisbane, California, United States -

Full Time

Start Date

Immediate

Expiry Date

29 Aug, 26

Salary

152630.0

Posted On

31 May, 26

Experience

2 year(s) or above

Remote Job

Yes

Telecommute

Yes

Sponsor Visa

Skills

Protein Expression, Baculovirus Expression, Mammalian Cell Culture, Recombinant Proteins, Sf9/Hi5 Cell Culture, HEK293 Cells, SDS-PAGE, Size Exclusion Chromatography, NanoDSF, Clone Design, Protein Purification, Affinity Chromatography, Ion Exchange Chromatography, Bacmid Generation, MOI Studies, Protein Characterization

Industry

Biotechnology

Description

Company Nurix Therapeutics is a clinical stage biopharmaceutical company focused on the discovery, development, and commercialization of targeted protein degradation medicines, the next frontier in innovative drug design aimed at improving treatment options for patients with cancer and inflammatory diseases. Powered by a fully AI-integrated discovery engine capable of tackling any protein class, and coupled with unparalleled ligase expertise, Nurix’s dedicated team has built a formidable advantage in translating the science of targeted protein degradation into clinical advancements. Nurix aims to establish degrader-based treatments at the forefront of patient care, writing medicine’s next chapter with a new script to outmatch disease. Position Summary: Research Associate III – Scientist I Protein Expression Nurix Therapeutics, Inc. is seeking a detail-oriented and experienced Protein Expression Research Associate to join the DEL and Protein Sciences team. This individual will work cross functionally with internal stakeholders to plan, coordinate, and execute all operational activities required for analytical scale and preparative scale expression of recombinant proteins in baculovirus and mammalian cell systems. Additionally, they will be responsible for documentation of expression conditions and results in internal databases and electronic lab notebook systems. This is an onsite role. Qualifications MS or equivalent with 2+ years of relevant experience, or Ph.D. This position requires 2+ years of experience working with insect cell lines and baculovirus expression of recombinant proteins. Additional experience working with recombinant protein expression in mammalian cells (e.g. HEK293) is a plus. Industry experience preferred with exemplified ability to overexpress milligram amounts of protein for structural and functional studies for multiple protein classes. Highly proficient in maintaining insect cell and mammalian cell cultures. Excellent knowledge and high degree of familiarity with all steps in baculovirus expression of recombinant proteins, including clone design, generation and purification of bacmids, raising and titering of baculovirus, conducting MOI studies, cell culture of Sf9 and/or Tni/Hi5 cells, and harvesting of biomass on the scale of 1 L – 20 L. Strong working knowledge of protein characterization steps, including SDS-PAGE analysis, size exclusion chromatography, and/or thermal stability (e.g. nanoDSF). Must be able to lift 50+ pounds comfortably. Familiarity with multiplexed/automated microscale purification technologies (e.g. magnetic beads) is desirable. Previous experience expressing multi-subunit protein complexes is a plus. Past experience troubleshooting difficult protein expressions for a variety of target proteins is a plus. Past experience purifying recombinant proteins (e.g. affinity chromatography, ion exchange, or size exclusion chromatography) is a bonus. Able to work in dynamic, fast-paced environment with experience prioritizing work across multiple projects. Detail-oriented with strong record keeping skills. Fit with Nurix Culture Strong communication skills and an effective team player. A respectful lab citizen who maintains an organized and clean working environment. Intellectually curious and highly motivated to learn. Salary Range: RA III $99,110 - $113,005 SRA $124,364 - $142,597 Scientist I $133,917 - $152,63 Location Brisbane, CA - Onsite Nurix Therapeutics, Inc. is committed to protecting and respecting your privacy and personal information, including information collected by Nurix when you apply for a job with Nurix or in the course of your employment with Nurix. By applying for a position at Nurix, you agree to our collection and use of personal information as described in our Privacy Policy (https://www.nurixtx.com/privacy-policy/).

Responsibilities

Plan and execute analytical and preparative scale expression of recombinant proteins using baculovirus and mammalian cell systems. Document expression conditions and results within internal databases and electronic lab notebooks.