• Job Type: Support Staff - Union
• Bargaining Unit: SSA
• Regular/Temporary: Regular
• End Date if Temporary:
• Hours Per Week: 35
• Standard Work Schedule:
• Building:
• Salary Range: $53,394.17- $53,394.17
  The salary of the finalist selected for this role will be set based on a variety of factors, including but not limited to departmental budgets, qualifications, experience, education, licenses, specialty, and training. The above hiring range represents the University’s good faith and reasonable estimate of the range of possible compensation at the time of posting.
  Position Summary
  The Department of Biochemistry and Molecular Biophysics is looking to hire Technician B to work in the laboratory of Dr. Sobolevsky. The candidate will be technical support staff for the Principal Investigator and other lab investigators, as needed.

Responsibilities

• Assist with general lab management tasks including keeping track of, organizing, and ordering supplies and organizing equipment maintenance, as needed.
• Usage of molecular biology tools to modify TRP channel coding sequences and introduce them into vectors for expression in bacterial, insect or mammalian cells.
• Utilization of different cell lines and expression systems to produce TRP channel protein on small and large scales.
• Usage of affinity and size-exclusion chromatography to purify TRP channel protein and characterize its behavior using Fluorescent-detection Size-Exclusion Chromatography (FSEC), SDS-PAGE and other biophysical and biochemical techniques.
• Making cryo-EM grids for screening at 200-kV transmission electron microscopes (TEMs) and collection at 300-kV TEMs.
• Cryo-EM data processing, structural model building and refinement.
• Analysis of structural data and design of mutations to test the molecular models.
• Electrophysiological recordings and calcium uptake measurements using fluorescent dyes to assess TRP channel function.
• Contribute to analysis of results and writing manuscripts.
• Perform other related duties and responsibilities as assigned/requested.

Minimum Qualifications

• Requires a bachelor’s degree in chemistry, biology, physics, or related science or equivalent combination of education, training and experience.

Preferred Qualifications

• Basic skills in molecular biology.
• Basic understanding of protein biochemistry and structure.
• Basic skills in protein analysis and purification (measurements of protein concentration, SDS PAGE, chromatographic techniques).
• Handling of eukaryotic cell culture and bacteria.
• Experience in fluorescent techniques (e.g., Fluorescence-detection Size-Exclusion Chromatography).
• Experience in cryo-EM sample preparation.
• Knowledge and usage of protein manipulation and analysis software (e.g., Coot, Chimera, Pymol).

Equal Opportunity Employer / Disability / Veteran
Columbia University is committed to the hiring of qualified local residents

• Assist with general lab management tasks including keeping track of, organizing, and ordering supplies and organizing equipment maintenance, as needed.
• Usage of molecular biology tools to modify TRP channel coding sequences and introduce them into vectors for expression in bacterial, insect or mammalian cells.
• Utilization of different cell lines and expression systems to produce TRP channel protein on small and large scales.
• Usage of affinity and size-exclusion chromatography to purify TRP channel protein and characterize its behavior using Fluorescent-detection Size-Exclusion Chromatography (FSEC), SDS-PAGE and other biophysical and biochemical techniques.
• Making cryo-EM grids for screening at 200-kV transmission electron microscopes (TEMs) and collection at 300-kV TEMs.
• Cryo-EM data processing, structural model building and refinement.
• Analysis of structural data and design of mutations to test the molecular models.
• Electrophysiological recordings and calcium uptake measurements using fluorescent dyes to assess TRP channel function.
• Contribute to analysis of results and writing manuscripts.
• Perform other related duties and responsibilities as assigned/requested