Italy
Project: “Exploring the role of non-coding RNAs in 3D chromatin Hubs” – Acronym: 3D-Hubs – Grant Agreement number: FIS-2023-02410 – CUP: J53C24004180001
Contract: Fixed-term contract, full time
Duration: up to 3 years
Location: Genova CHT@Erzelli

ESSENTIAL REQUIREMENTS

• PhD in Cellular/Molecular Biology, Biochemistry or similar disciplines
• Extensive experience in a molecular biology, biochemistry, genetics or cell biology laboratory
• Previous experience in producing NGS data such as ChIP-seq, RNA-seq, ATAC-seq etc
• Experience with cell culture maintenance and complementary techniques (e.g. FACS)
• Familiarity with CRISPR technologies
• Good publication record
• Strong command of spoken and written English
• Ability to work independently and collaboratively in a highly interdisciplinary environment

ADDITIONAL SKILLS

• Experience in 3D chromatin conformation assays (e.g. Hi-C, Hi-ChIP etc)
• Previous research experience in the RNA field
• Previous research experience in the stem cells field
• Ability to perform genomic and epigenomic editing in stem cells using CRISPR-(d)Cas technologies

You will be working in a multicultural and multi-disciplinary group, where junior and senior scientists collaborate, each with their expertise, to carry out a scientific activity with a shared research goal.
The “3D Chromatin Conformation and RNA Genomics” Research Unit is coordinated by Dr. Dafne Campigli Di Giammartino and aims at understanding how non-coding elements (such as enhancers and non-coding RNAs) and their epigenetic/epitranscriptomic modifications regulate 3D genome architecture and gene expression. To address this question, we use cutting-edge chromatin conformation assays (e.g.Hi-C, Hi-ChIP etc) in conjunction with other -omics techniques (e.g. ChIPseq, RNA-seq etc.) and in combination with CRISPR-based genetic and epigenetic engineering tools in mouse stem cells as well as in human tumor stem cells.

Within the research team, your main responsibilities will be:

• Using advanced molecular biology techniques for the manipulation of RNA, DNA and proteins in the area of 3D chromatin conformation, stem cells and non-coding RNAs
• Collaborating with other lab members to implement new technologies based on CRISPR/Cas platforms and biochemical assays for the characterization of the 3D chromatin architecture
• Supervising and mentoring junior members of the team
• Recording experimental data performed in the laboratory in both paper and electronic format (e.g. benchling)
• Writing research and reviewing articles as well as assisting in generating preliminary data and figures for grant proposals