SKILL DEVELOPMENT AND COLLABORATION:

• Develop and refine technical skills in collaboration with colleagues.
• Participate in weekly reviews of primary data with the supervisor.
• Collaborate with team members to support shared research goals and perform additional duties as directed by the Principal Investigator or senior staff.

SKILLS

• Cell line and primary cell culture experience.
• ELISA or bead-based immunoassays.
• Nucleic acid extraction, RT-PCR, PCR, Real-time qPCR, and agarose gel electrophoresis.
• Cell isolation using density gradient centrifugation, FACS and/or MACS-based methods.
• Flow cytometer operation, surface/intracellular marker staining and flow data analysis with FlowJo software (plus but not required)
• Mouse handling, tracking, sexing, anesthesia, blood withdraw, injection (tail vein intravenous, intraperitoneal, footpad and subcutaneous), and dissection. (plus but not required).
• Must be team oriented and able to work in a highly collaborative and interactive work environment to complete complex tasks.
• Must be able to manage multiple and rapidly changing priorities and have the ability to quickly learn new skills.
• Must be detail-oriented and well organized.

EDUCATION/TRAINING:

Work requires a bachelor’s degree in botany, biology, zoology, psychology or other directly related scientific field.

EXPERIENCE:

Work requires two years of research experience. A related master’s degree may offset required years of experience on a 1:1 basis, e.g., a two year master’s degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE.
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Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.
Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.