Research Assistant 3

at  Case Western Reserve University

QUALIFICATIONS

Experience: 3 to 5 years of experience required.
Education: Bachelor’s degree in Biochemistry, Molecular Biology, or a related field required.

REQUIRED SKILLS

• Thoroughly understands commonly used concepts, practices, and procedures within molecular biology and microbiology.
• Experience in molecular biology techniques, excellent aseptic technique, and general skills common to a microbiology lab are necessary.
• Strong documentation and organization skills with the ability to keep accurate records of all work performed.
• Good analytical, statistical, and presentation skills.
• Proficient computer skills, particularly using Adobe Illustrator, Photoshop, and graphing software such as Origin or Prism. Skills in single-cell data analysis using R or Python and basic knowledge of Linux and bash are encouraged.
• Demonstrated willingness to learn new techniques with instruction.
• Willingness to work in a biosafety level 2+ setting.
• Relies on instructions and pre-established guidelines to perform the functions of the job.
• Ability to interact with colleagues, supervisors, and customers face to face.
• Ability to operate laboratory equipment. This includes qPCR and ddPCR, fluorescent microscopes, flow cytometers, cell counters, and routine molecular biology laboratory equipment such as centrifuges, scales, pipettes, etc.
• Demonstrated history of successful support, education, and advocacy for all students, aligned with the values, mission, and messaging of the university, while adhering to the staff policy on conflict of commitment and interest.
• Ability to meet consistent attendance.

ESSENTIAL FUNCTIONS

• Coordinate laboratory/ research activities. This requires performing experiments using molecular biology technologies, single-cell transcriptomics, and basic bioinformatics using primary cell models. Perform immunofluorescence flow cytometry and microscopy. (10%)
• Carry out complex research assignments of a non-routine nature. This includes single-cell capture and library preparation using BD Rhapsody or a similar single-cell platform (scRNAseq, ABseq, scATACseq), the preparation of total RNA and DNA from cells, performing highly reproducible ddPCR and RT-qPCR analysis of samples containing HIV or reporter viruses. (50%)
• Devise new protocols and techniques for research projects involving a high degree of skill and training. This includes methods for multi-omic analyses of cells and new methods to detect and sequence HIV RNA and proviral DNA. May supervise students and technicians. (10%)
• Evaluate adequacy of techniques; study and test new procedures; and analyze data. This will include assisting in data and bioinformatic analyses using R or Python-based programs. Maintain detailed laboratory records, prepare figures, prepare data for deposition by NIH data-sharing policies, and assist in writing related to experiments. (18%)
• Work closely with the principal investigator. This includes regular individual meetings every 3 weeks and many additional ad hoc meetings with project team members to discuss priorities, goals, experimental design, data management, data analysis strategy and interpretation of results. Participate in general laboratory meetings and read applicable literature. May co-author research projects. (6%)
• Maintain lab supplies and inventory (ordering, stocking, preparing solutions/media). Maintain general lab environment, including, but not limited to, equipment maintenance and scheduling repairs, organization, and cleanliness of the lab. Maintain and ensure compliance with biosafety and laboratory regulations. May monitor the laboratory budget. May supervise students and technicians. (6%)