Postdoctoral Research Associate (Fixed Term)

at  University of Cambridge

POSTDOCTORAL RESEARCH ASSOCIATE (FIXED TERM): GENETIC INTERROGATION OF THE UBIQUITIN-PROTEASOME SYSTEM

We are looking for a talented and motivated post-doctoral scientist to join the laboratory of Dr. Richard Timms based in the Cambridge Institute of Therapeutic Immunology and Infectious Disease (CITIID).
The Timms lab applies the latest genetic technologies to identify novel functions for human genes. Our work is focused on the ubiquitin-proteasome system, where we seek to (1) identify substrates for E3 ubiquitin ligases, (2) characterise the molecular features that enable selective substrate recognition, and (3) explore how these processes are corrupted in the context of viral infection and autoimmune disease. We exploit a range of high-throughput genetic screening techniques to uncover novel pathways regulated by the ubiquitin system. We measure the stability of GFP-tagged proteins by performing expression screens in human cells, using either a human ORFeome library comprising ~14,000 barcoded human ORFs or custom libraries generated through microarray-based oligonucleotide synthesis, and identify the cellular machinery involved by combining these expression screens with loss-of-function CRISPR/Cas9 screens. Detailed follow-up of individual pathways of interest is achieved through a variety of standard genetic and biochemical approaches. Recent publications relevant to this position include:
Timms RT et al. (2023) Defining E3 ligase¿substrate relationships through multiplex CRISPR screening. Nature Cell Biology, 25: 1535¿1545. Timms RT and Koren I (2020) Tying up loose ends: the N-degron and C-degron pathways of protein degradation. Biochem Soc Trans, 48 (4): 1557¿1567. Timms RT et al. (2019) A glycine-specific N-degron pathway mediates the quality control of protein N-myristoylation. Science, 365 (6448): eaaw4912. Koren I, Timms RT et al. (2018) The Eukaryotic Proteome Is Shaped by E3 Ubiquitin Ligases Targeting C-Terminal Degrons. Cell, 173 (7): 1622-1635. For more information, visit https://www.timmslab.com The successful candidate will be passionate about genetics, eager to tackle difficult problems and be excited to develop novel experimental approaches to study gene function. Prior experience with mammalian cell culture, lentiviral transduction, CRISPR genome editing, flow cytometry, next-generation sequencing and bioinformatic data analysis (preferably in Python) would be advantageous, but an eagerness to learn and develop innovative methods is the only critical requirement as we will teach you all of the necessary skills. You will play a key role in a small team, and so a friendly and collegial attitude is crucial.
Appointment at Research Associate level is dependent on having a PhD, or an equivalent research doctorate. Those who have submitted but not yet received their PhD will be appointed at Research Assistant level, which will be amended to Research Associate once PhD has been awarded.
For further details or informal enquiries please contact Richard Timms via email at rtt20@cam.ac.uk
Fixed-term: The funds for this post are available for 1 years in the first instance.
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