Research Technician II

at  Fred Hutchinson Cancer Center

Overview:
Fred Hutchinson Cancer Center is an independent, nonprofit organization providing adult cancer treatment and groundbreaking research focused on cancer and infectious diseases. Based in Seattle, Fred Hutch is the only National Cancer Institute-designated cancer center in Washington.
With a track record of global leadership in bone marrow transplantation, HIV/AIDS prevention, immunotherapy and COVID-19 vaccines, Fred Hutch has earned a reputation as one of the world’s leading cancer, infectious disease and biomedical research centers. Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy and related services, and network affiliations with hospitals in five states. Together, our fully integrated research and clinical care teams seek to discover new cures to the world’s deadliest diseases and make life beyond cancer a reality.
At Fred Hutch we value collaboration, compassion, determination, excellence, innovation, integrity and respect. These values are grounded in and expressed through the principles of diversity, equity and inclusion. Our mission is directly tied to the humanity, dignity and inherent value of each employee, patient, community member and supporter. Our commitment to learning across our differences and similarities make us stronger. We seek employees who bring different and innovative ways of seeing the world and solving problems. Fred Hutch is in pursuit of becoming an anti-racist organization. We are committed to ensuring that all candidates hired share our commitment to diversity, anti-racism and inclusion.
The Nelson research group (https://research.fredhutch.org/peternelson/en.html) at the Fred Hutch Cancer Center has an opening for a Research Technician II for a scientist interested in contributing to efforts to develop new treatments for advanced cancer. The Nelson group is embedded within a highly collaborative research environment and is comprised of scientists with a diverse range of skills and expertise. The successful applicant will apply novel experimental approaches to systematically understand how cancers resist current therapies and to develop new models that take advantage of high-throughput technologies that include spatial profiling and genetic/compound screens. There is ample opportunity to learn new skills and for contributing to research projects.
Our research team is seeking an outstanding Research Technician to work on developing and implementing new therapeutics with the goal of translating these discoveries to improve the outcomes and lives of patients with advanced cancer. The ideal candidate will have a strong interest in cancer biology, immunology, mouse modeling and application of cutting edge technologies. The technician will be responsible for performing standard and investigative laboratory work, including, following detailed, time-sensitive and challenging protocols to generate organoids, mouse models and cell/tissue transplant experiments, conducting drug studies, conducting and quantifying tumor measurements by a variety of in vivo and ex vivo imaging approaches, and by conducting and quantifying tissue-based immunohistochemistry and spatial profiling.
Responsibilities:

Key responsibilities will include (skills can be taught):

• Working collaboratively with other lab members to execute complex experimental studies of cancer therapeutics including assessments of tumor responses in vitro and in vivo.
• Maintain and perform husbandry and genotyping of murine/mouse colonies of patient derived xenografts as well as maintain associated databases.
• Performing cell-culture experiments, molecular biology, and preparation of next-generation sequencing libraries.
• Histology, cell culture, maintain mice colonies, imaging mice, stocking reagents
• Tag, weigh and record data used for tracking purposes and administer drugs/reagents per guided protocols, including the harvest of cancer metastasis tumors
• Perform, collect and analyze flow cytometric assays
• IVIS and ultrasound imaging
• Attending lab meetings and presenting results.
• Reading relevant scientific literature.
• Assisting with general lab upkeep and maintenance.
• Optionally (depending on skills and interests) assisting with computational data analysis and the preparation/authorship of manuscripts/research results

Qualifications:

MINUMUM QUALIFICATIONS

• Bachelor’s degree in related scientific field.
• Minimum 2 years previous experience work in a laboratory in a related field of study.
• A Master’s degree can substitute for 2 years of post-graduation lab experience.

PREFERRED QUALIFICATIONS

• Experience with mouse experiments such as studies of xenografts and/or husbandry preferred
• Molecular tissue culture, DNA/RNA isolation, sequencing, and molecular analysis experience a plus
• Experience/familiarity with R or other programming languages is an asset
• Superior organization and communication skills
• Assay validation and optimization experience
  We will consider applicants from diverse backgrounds and with different levels of experience. We will also base our evaluation on your specific interest and motivation for this position. Accordingly, in your application please include a cover letter that describes your interest and motivation related to the recent research in our lab (see papers here: (https://research.fredhutch.org/peternelson/en/publications.html) along with your CV and contact information for at least two references.
  A statement describing your commitment and contributions toward greater diversity, equity, inclusion, and antiracism in your career or that will be made through your work at Fred Hutch is requested of all finalists.
  The hourly pay range for this position is from $25.71 to $36.60 and pay offered will be based on experience and qualifications.
  Fred Hutchinson Cancer Center offers employees a comprehensive benefits package designed to enhance health, well-being, and financial security. Benefits include medical/vision, dental, flexible spending accounts, life, disability, retirement, family life support, employee assistance program, onsite health clinic, tuition reimbursement, paid vacation (12-22 days per year), paid sick leave (12-25 days per year), paid holidays (13 days per year), paid parental leave (up to 4 weeks), and partially paid sabbatical leave (up to 6 months).
  Our Commitment to Diversity: We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We are committed to cultivating a workplace in which diverse perspectives and experiences are welcomed and respected. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We are an Affirmative Action employer. We encourage individuals with diverse backgrounds to apply and desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to Human Resources at hrops@fredhutch.org or by calling 206-667-4700

• Working collaboratively with other lab members to execute complex experimental studies of cancer therapeutics including assessments of tumor responses in vitro and in vivo.
• Maintain and perform husbandry and genotyping of murine/mouse colonies of patient derived xenografts as well as maintain associated databases.
• Performing cell-culture experiments, molecular biology, and preparation of next-generation sequencing libraries.
• Histology, cell culture, maintain mice colonies, imaging mice, stocking reagents
• Tag, weigh and record data used for tracking purposes and administer drugs/reagents per guided protocols, including the harvest of cancer metastasis tumors
• Perform, collect and analyze flow cytometric assays
• IVIS and ultrasound imaging
• Attending lab meetings and presenting results.
• Reading relevant scientific literature.
• Assisting with general lab upkeep and maintenance.
• Optionally (depending on skills and interests) assisting with computational data analysis and the preparation/authorship of manuscripts/research result