Research Technician II - Pediatric Genetics

at  Washington University in St Louis

POSITION SUMMARY

The Greer lab is interested in how non-genetic information can be transmitted across generations and how non-genetic information regulates complex phenotypes. An increasing number of complex phenotypes, such as physical appearance, energy metabolism, psychological state, and longevity, have recently been shown to be regulated, in part, by epigenetic information. Epigenetics describes how gene expression changes occur without changes to the DNA sequence. Proteins, RNA molecules, or chemical modifications to histones or DNA can induce these epigenetic changes. How this information, which is not directly coded in our DNA, is passed from generation to generation is still unknown. Understanding the molecular determinants of stable epigenetic memory will provide insight into how environmental changes can affect the health and lifespan of not only the individual who experiences them, but also of their progeny. Our goals are to identify epigenetic inheritance phenotypes and to elucidate the mechanisms behind their transmission across generations. We also aim to understand how epigenetic mis-regulation can cause developmental defects and disease.
In the Greer lab we use the nematode C. elegans, the social amoeba Dictyostelium, mice, and mammalian tissue culture systems to study how non-genetic information can be transmitted across generations as well as to understand the role of non-genetic information in regulating a variety of biological processes. We use a wide variety of genetic, biochemical, and molecular techniques to decipher these complex question. Some experiments would entail worm, Dictyostelium, or mouse husbandry, extracting gDNA and proteins from worm and tissue samples, running dot blots and western blots, purifying proteins and testing their enzymatic activity. The Greer lab strives to be a place of fun, collegiality, and collaboration. While some research experience is a big plus it is not required.
Assists with the technical aspects of studies and experiments, including documentation and preparation of materials.

EDUCATION:

Bachelor’s degree or combination of education and/or experience may substitute for minimum education.

WORK EXPERIENCE:

No specific work experience is required for this position.

SKILLS:

Not Applicable

EDUCATION:

No additional education beyond what is stated in the Required Qualifications section.

WORK EXPERIENCE:

No additional work experience beyond what is stated in the Required Qualifications section.

SKILLS:

Communication, Computer Software Programs, Detailed Reports, Interpersonal Relationships, Laboratory Instrumentation, Laboratory Research, Organizing Files, Working Independently

• Assists with research studies, experiments and assays, including collection of data, preparation of solutions and setup and maintenance of equipment.
• Performs data entry and maintains data files on research.
• Prepares tissue cultures, reagents, probe tests, etc.
• Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions.
• Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures.
• Assists with general lab maintenance and cleaning.
• Performs other duties as assigned.