Research technician to improve immunotherapy against cancer

at  Radboudumc

2 years with prospect of extension We are looking for an enthusiastic and motivated technician to contribute to the development of new immune therapies for cancer patients. As a research technician, you work alongside PhD students to improve immunotherapy against cancer. In this translational project, funded by KiKa, you focus on enhancing allogeneic Natural Killer (NK) cell therapy for (pediatric) acute myeloid leukemia (AML), and contribute to translating this research into the clinic.
Within this project, you are responsible for collecting umbilical cord blood from the operating room and isolating CD34+ stem cells from this blood. Through a culturing process, you expand and differentiate these CD34+ stem cells into large quantities of NK cells. These NK cells are used for functional in vitro and in vivo experiments. Additionally, you work on improving the specificity, activity, and expansion of these NK cells through viral transduction of chimeric antigen receptors (CAR). These CARs are specifically targeted against proteins that are highly expressed on AML cells, allowing these CAR NK cells to operate more precisely.
Do you want to contribute to the development of new cancer therapies? Come and join our team!

Tasks & Responsibililties

• Collecting and processing umbilical cord blood from the operating room and delivery rooms.
• Sterile isolation and culturing of primary cells and cell lines.
• Cloning CAR constructs.
• Producing plasmids and (lenti and retro) viruses.
• Performing transfections and transductions.
• Conducting flow cytometry and functional assays.
• Independently conducting experiments.
• Reporting results in meetings and through presentations to the department.
• Ensuring accurate documentation.

At Radboud university medical center, you build on your future. We are committed to providing the best care, education, and research. And we are true to our word, because we help you develop and seize opportunities and give you the room to grow. As an employer, we believe that employees should feel vital and happy at work in all stages of life. We are also committed to creating a healthy and safe working environment. Our employment conditions contribute to that. What we offer:

• A gross monthly salary between € 3.401 and € 4.816 (scale 8M) for full-time employment (36 hours per week). You will also receive an 8% holiday allowance, an 8.3% end-of-year bonus, and, depending on the position, an allowance of 47% to 72% for working irregular hours.
• 172 vacation hours per year based on a 36-hour working week. (As of 1 January 2025, you will receive 176 vacation hours).

You will work in the Immunotherapy research group of the Laboratory of Hematology, Department of Laboratory Medicine, Radboudumc, Nijmegen, the Netherlands. We focus on the characterization and modulation of anti-tumor immune responses in cancer patients.
Our translational research program encompasses four intermingled themes: 1) development of novel strategies to boost antitumor immunity after allogeneic stem cell transplantation, 2) co-signaling pathways involved in tumor immune escape, 3) DC-based vaccination strategies against hematological cancers, and 4) T and NK cell-based immunotherapy against leukemia, multiple myeloma and ovarian cancer. Our research is embedded in the Radboud Research Institute.
The studies indicated in this vacancy will be performed in close collaboration with clinicians of the Department of Hematology at the Radboudumc and the Prinses Máxima Center. We provide a stimulating work environment within a group of enthusiastic non-clinical and clinical researchers with a strong national and international research network and track record.

• Collecting and processing umbilical cord blood from the operating room and delivery rooms.
• Sterile isolation and culturing of primary cells and cell lines.
• Cloning CAR constructs.
• Producing plasmids and (lenti and retro) viruses.
• Performing transfections and transductions.
• Conducting flow cytometry and functional assays.
• Independently conducting experiments.
• Reporting results in meetings and through presentations to the department.
• Ensuring accurate documentation