Research Technologist

at  Johns Hopkins University

The Department of Neurology is seeking a Research Technologist for a molecular virology (HIV-1, biosafety level 2) laboratory. The Research Technologist will utilize a solid foundation in basic laboratory techniques, molecular biology and primary cell culture requiring a high level of rigor and reproducibility. The Research Technologist will exercise independent judgment while participating in the planning of experiments and interpreting results. The projects require care in data collection, preparation of reports and interfacing with sponsors. The work requires occasional irregular work hours consistent with research needs related to in vivo studies with mice.

ADDITIONAL KNOWLEDGE, SKILLS & ABILITIES

• Willing to learn Isolation of PBMCs and culture of primary human cells; sterile technique.
• Willing to learn and implement new technologies.
• Ability for self-management, integrity and professionalism.
• Work effectively as a team-member and exhibit excellent interpersonal, organizational, and self-management skills.

MINIMUM QUALIFICATIONS

• Bachelor’s Degree in Biology, Chemistry or related field.
• Willing to work under Biohazard Level 2 Safety conditions with HIV-1.
• Flexibility to occasionally work irregular hours consistent with research needs.

Preferred Qualifications

• Master’s Degree in Biological Sciences preferred.
• Some related post-undergraduate work experience.
• Experience and or interest in learning how to conduct single-cell genomics and computational analyses.

Classified Title: Research Technologist
Role/Level/Range: ACRO40/E/02/CC
Starting Salary Range: $15.70 - $26.25 HRLY (Commensurate with experience)
Employee group: Full Time
Schedule: M-F 8:30 am - 5:00 pm
Exempt Status: Non-Exempt
Location: School of Medicine Campus
Department name: SOM Neuro Neuroimmunology
Personnel area: School of Medicine

• Mouse breeding and husbandry.
• Handling and performing blood draw and injections on mice infected with viral pathogens.
• Isolation of peripheral blood lymphocytes and monocytes from human blood.
• Perform in vitro infection studies with HIV-1.
• Perform general molecular biology techniques such as cloning, qPCR, and Western blotting.
• Perform specialized techniques including multicomponent single-cell labeling, confocal and high-content microscopy, and multi-color flow cytometry.
• Provide training to other individuals who may be assigned to the laboratory on a regular or temporary basis.