Scientist II - Protein Expression & Purification

at  Revvity

JOB SUMMARY

This position is responsible for developing functional protein products by using cutting-edge protein expression and purification technologies. The duties include expressing and producing high-quality recombinant proteins for use as a research reagent, working with cloning and cell culture teams on protein expression, purification, and characterization from both prokaryotic or eukaryotic expression platforms for recombinant protein products.
He/she should possess excellent technical, troubleshooting, data collection/presenting, and project prioritizing skills to ensure on-time project closure of quality product development.
Strong ability to self-start, adapt into system practices and also align your creative development design with company’s quality regulation. This position offers an excellent opportunity for technology exploration, scientific knowledge expansion, leadership development, and career growth in a leading biotechnology company.

PREFERRED QUALIFICATIONS – EDUCATION AND EXPERIENCE

• Experience in signaling factors (i.e. Wnt proteins, interleukins, etc), cell surface proteins/receptors (i.e. Frizzled, notch, etc) or structural biology
• Technical skills including recombinant protein design, prokaryotic protein purification, refolding, protein solubility, and buffer formulation, conjugation, or protein characterization
• Prior working experiences under ISO13485/GMP environment
  The base salary range for this full-time position is $90,000 -$110,000. This range reflects the minimum and maximum target for a new hire in this position. The base pay actually offered to the successful candidate will take into account internal equity, work location, and additional factors, including job-related skills, experience, and relevant education or training. Your recruiter can share more about the specific salary range for your preferred location during the hiring process.
  Please note that base pay is only one part of our total compensation package and is determined within a range. This range allows for the successful candidate to have an opportunity to progress within the position and develop at our company. This base pay range does not take into account bonuses, equity, or other benefits which may be applicable and are dependent on the level and position offered.

Minimum Qualifications – Education and Experience

• B.S. with generally more than 12 yrs of relevant experience OR
• M.S. with generally more than 8 yrs of relevant working experience OR
• Ph.D. with generally more than 4-6 years of post-doctoral training or relevant working experiences

Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

• Familiar with recombinant protein product expression platforms of various protein families such as cytokine, growth factor, enzyme, and ligand receptors from different research areas cancer, immunology, neuroscience etc.
• Develop and run protein purification schemes using various chromatography methods on various platforms (i.e. AKTA FPLC, HPLC).
• Implement protein production activities from expression to technical transfer under ISO9001:2008/ISO13485:2016 standards.
• Participate in product design stage review and provide quality records to ensure traceability and risk control for product release.
• Produce immunogens from prokaryotic or eukaryotic expression systems to facilitate antibody product development.
• Own assigned product family and is responsible to drive projects to meet monthly, quarterly, and yearly team goals for product development.
• Lead troubleshooting and optimization on protein expression, homogeneity, and stability of developing and released products.
• Support to execute and validate different protein chemistry protocols such as protease cleavage and conjugation
• Actively involved in defining, designing, and developing relative biological methods for product validation with application teams for both new product development and current product improvement projects.
• Facilitate Manufacturing Group for purification/QC workflow development and SOP optimizing/ troubleshooting in various production stages including expression constructs, culture systems, purification schemes, formulation, and stability issues.
• Establish, coordinate, and communicate project timelines and deliverables with internal and external product development teams.
• Responsible for personnel training, goal setting, and performance management.
• Maintain a current knowledge of protein chemistry, synthetic biology, and scientific literature. Identify and validate.
• Prepare and present experimental results following Good Document Practices.
• Assist and train other research associates to meet the company performance standard
• Other project responsibilities may be required.

Minimum Qualifications – Education and Experience

• B.S. with generally more than 12 yrs of relevant experience OR
• M.S. with generally more than 8 yrs of relevant working experience OR
• Ph.D. with generally more than 4-6 years of post-doctoral training or relevant working experiences.