Scientist III, Cell Biology

at  Thermo Fisher Scientific

KNOWLEDGE, SKILLS, ABILITIES

• Experience with primary, stem and/or immune cells
• Isolation and purification of various primary blood cell types using manual and/or automated methods
• Experienced with planning and execution of reagent-based transfection and electroporation based delivery experiments
• CRISPR based assay development for both knock-out and knock-in optimization
• Flow Cytometry experience (panel analysis and optimization)
• Molecular biology techniques (primer and construct design, PCR, NGS, etc.)
• Standard laboratory math calculations and solutions/media preparation
• Experience in a biotechnology commercial environment and product development is highly preferred
• Significant level of independence and strong teamwork

EDUCATION REQUIREMENTS

• The ideal candidate will possess a PhD degree with 1-2 years of relevant experience, or a M.S. degree with 4-5 years of relevant experience preferably within Immunology and/or Cell Biology

• Design, perform and troubleshoot cell transfection experiments using electroporation and other methods with various types of payloads
• Isolate and maintain culture of multiple immune cells, stem cells and cancer cell models
• Optimize protocols for genetic modification of various cell types
• Setup and perform sophisticated flow cytometry assays with proper compensation
• Maintain detailed and organized notebook and experiment write-ups
• Deliver on goals according to defined plans and timelines
• Apply Design of Experiment methodology to promptly plan experiments and analyze data
• Directly interact with senior R&D leadership, product management and project management to develop and carry out project plans that support product development initiatives
• Present data and findings to cross-functional teams of peers and managers
• Prioritize workload across multiple projects
• Effectively collaborate with team members and cross-functional
• Work independently in a fast-paced project-based environment