Scientist/Senior Scientist

at  Evox Therapeutics

KEY ACCOUNTABILITIES

The candidate will work in a collaborative matrix structure, working closely with the R&D team. The key responsibilities will include:

• Engineer extracellular vesicles to carry gene editing modalities to be delivered to CNS.
• Perform extensive analysis of the product for quantification of editor/guides using various analytical techniques.
• Validate the Genome Ediitor delivery in vitro, using relevant cell lines and analytical tools for the gene editing read-outs (testing uptake, distribution, efficacy, specificity)
• Identify relevant in vitro models and perform bridging studies to map the translatability of the product from in vitro to in vivo
• Analyse, summarise, and document experimental results, as well as being able to communicate them effectively in meetings
• Test and troubleshoot new assays and techniques and propose next steps

ESSENTIAL SKILLS:

• Expertise in cell culture manipulation, maintenance, and transfection, along with molecular characterization of edited cells
• Proficiency in molecular biology techniques, including DNA/RNA purification, DNA sequence analysis, PCR, cloning, qPCR, FACS, ICC, and Western blotting
• Hands-on experience with gene editing technologies or experience working with extracellular vesicles (EVs)
• Experience using sequence manipulation software (e.g., Vector NTI, SnapGene)
• Eagerness to learn and explore emerging scientific fields
• Ability to work effectively in a fast-paced, dynamic environment and meet deadlines

BENEFICIAL SKILLS:

• Background knowledge in neuroscience or neurological diseases
• Hands-on experience with pre-clinical models in the CNS field
• Previous experience in an industry setting
• Knowledge of exosome or lipid nanoparticle-based therapeutics
• Experience working with iPSC-derived neurons
• Familiarity with gRNA design and off-target mapping tools

MAIN PURPOSE OF JOB

The role will be an integral part of the Research Department, playing a key role in advancing Genome Editing technology, delivered by exosomes, towards pre-clinical and clinical studies. The successful candidate will apply various loading strategies to deliver Genome Editing therapeutics, contributing to the development of a new class of biotherapeutic products. Additionally, they will be responsible for evaluating the uptake, distribution, efficacy, and specificity of the loaded material in disease-relevant models.

The candidate will work in a collaborative matrix structure, working closely with the R&D team. The key responsibilities will include:

• Engineer extracellular vesicles to carry gene editing modalities to be delivered to CNS.
• Perform extensive analysis of the product for quantification of editor/guides using various analytical techniques.
• Validate the Genome Ediitor delivery in vitro, using relevant cell lines and analytical tools for the gene editing read-outs (testing uptake, distribution, efficacy, specificity)
• Identify relevant in vitro models and perform bridging studies to map the translatability of the product from in vitro to in vivo
• Analyse, summarise, and document experimental results, as well as being able to communicate them effectively in meetings
• Test and troubleshoot new assays and techniques and propose next step