Research Technologist

at  Johns Hopkins University

The Department of Genetic Medicine is seeking a Research Technologist who will be responsible for sample handling, planning and executing molecular genetics tests (i.e., genotyping or sequencing) on large numbers of DNA samples.

SPECIAL KNOWLEDGE, SKILLS, AND ABILITIES

• Basic understanding of molecular biology and genetics.
• Ability to safely operate scientific laboratory equipment.
• Able to work flexible hours beginning as early as 6:00 a.m. and/or ending as late as 6:00 p.m.
• Superior organizational skills for time and data management.
• Ability to function well in a team production setting.

Machines/equipment used

• Centrifuges, Illumina BeadLab system (Tecans, iScans, TeFlow), Covaris, BioAnalyzer, LabChip GX Touch, Qubit, Nanodrop instruments, Illumina NovaSeq, MiSeq and iSeq, thermal cyclers, robotic and manual liquid handlers, common laboratory equipment, Macintosh, PC computers, UNIX servers, Oracle database.

PHYSICAL REQUIREMENTS

• Sitting in a normal seated position for extended periods of time.
• Reaching by extending hand(s) or arm(s) in any direction.
• Finger dexterity to manipulate objects with fingers rather than with whole hand or arm, for example, using a keyboard.
• Communication skills using the spoken word.
• Ability to see and hear within normal parameters.
• Ability to move about workspace. Lift, carry, move supplies, computers, etc.

MINIMUM QUALIFICATIONS

• Bachelor’s Degree in Biology, Chemistry or related field.

PREFERRED QUALIFICATIONS

• Six months of post-undergraduate experience in a comparable lab or significant research project, not related to normal course requirements.
  Classified Title: Research Technologist
  Role/Level/Range: ACRO40/E/02/CC
  Starting Salary Range: $15.50 - $26.00 HRLY ($43,160 targeted; Commensurate with experience)
  Employee group: Full Time
  Schedule: 8.5 hours between hours of 6 AM - 6 PM Mon-Friday
  Exempt Status: Non-Exempt
  Location: School of Medicine Campus
  Department name: SOM Genetics Ctr for Inhrtd Dis Res CIDR
  Personnel area: School of Medicine

• Barcode and enter sample associated information into the database.
• Transfer DNA samples into 96-well plate format.
• Receive and prepare deepwell DNA stock plates and create working DNA plates.
• Determine concentration and volume of DNA samples using various instruments.
• Dilute samples to working concentrations.
• Maintain and monitor inventory of all in-house DNA samples and reagent supplies.
• Create master mixes; Set up polymerase chain reactions; trouble-shoot such reactions.
• Operate and troubleshoot robotic liquid handlers.
• Prepare and complete SNP genotyping assays including, but not limited to, Illumina assays and workflows.
• Prepare samples for targeted capture of specific regions of DNA for sequencing experiments. This includes library preparation and capture techniques.
• Prepare and complete Next-Gen sequencing assays including, but not limited to, Illumina NovaSeq workflows.
• Perform quality control checks of data using various software, including, but not limited to, GenomeStudio, Spotfire and other in-house QC metrics reports.
• Maintain timely and accurate laboratory records (electronic and written).
• Collect, organize, interpret and submit experimental data to database and supervisors.
• Perform general lab maintenance duties.
• Perform validation tests of lab reagents.
• Participate in committees that function to improve processes.
• Communicate effectively to supervisor and team members.
• Perform miscellaneous related duties as assigned.