Scientist (f/m/d) Membrane Proteins

at  Nuvisan GmbH

REQUIREMENTS

• Completed university studies and Ph.D in the fields of biochemistry, biology, chemistry or related fields, 2 years postdoc experience or professional experience, preferably in the field of protein science appreciated.
• Publications as evidence of scientific success.
• Proven experience in purification of membrane proteins (e.g. GPCRs, ion channels, transporter).
• Experience with chromatography systems (e.g. ÄKTA) and processes in the field of protein production.
• Experience in structure determination and data processing with Cryo-EM.
• Experience with Nanobody screening is desirable.
• Experience with biochemical and biophysical assays with membrane proteins (NanoDSF, SPR, CPM, etc.) is desirable.
• Talent for coordination and organization as well as communication and team skills.

• The main task is the production of recombinant membrane proteins for biochemical and biophysical assays and for structure determination (X-Ray, CryoEM) in a scientific working group
  o Design of expression constructs using in silico tools
  o Expression of membrane proteins in different systems (mammalian, insects).
  o Purification of membrane proteins.
  o Reconstitution of membrane proteins in Nanoparticles (NanoDiscs, Liposomes)

o Biophysical assays with membrane protein to characterize binding of interaction partners

• Evaluation and interpretation of the data obtained, as well as their documentation and presentation
• Written and oral presentations in English for various target groups as well as creating project plans in collaboration with colleagues and customers
• Organization of your own work and the processes in the function including maintenance and further development of the infrastructure (hardware and IT) for the processing of the research projects
• Evaluation and implementation of new technologies for the optimal handling of the given tasks