Senior Scientist: ELISA, PCR, Cell Culture - Labs FSP

at  Thermo Fisher Scientific

JOB DESCRIPTION

Thermo Fisher’s clinical research business is a leading global contract research organization and world leader in serving science. We are passionate, deliberate, and driven by our mission – to enable our customers to make the world healthier, safer, and cleaner.
Within our Analytical Services team, we have a functional service provider solution, which is a unique partnership that allows our customers to leverage the experience of our staff, while allowing you, the employee, to gain direct experience working onsite at a pharmaceutical/ biopharmaceutical company, all while maintaining full-time benefits.

PREFERRED QUALIFICATIONS:

• Excellent communication skills and ability to effectively collaborate within multiple project workstreams
• Critical thinking skills and ability to efficiently manage changes in priorities and timelines
• Attention to detail and meticulous execution of project-related tasks
• Plasmid and viral vector cloning, qPCR/ ddPCR, wet lab next-generation sequencing (NGS)
• Experience with FACS analyser/single cell sorter
• Experience with CRISPR or other genomic editing tools
• Applies detailed fundamental knowledge of own specialty area or scientific discipline into solving process development problems
• Experience with Mammalian Cell Culture
• Identifies and prioritizes solutions using knowledge gained through formal education, experience, and sound judgment
• Fully understands the rationale and hypotheses for experimental designs

EDUCATION AND EXPERIENCE:

Bachelor’s degree in molecular biology, cell biology, virology, or other vaccinology-related discipline or equivalent and relevant formal academic / vocational qualification
Previous experience that provides the knowledge, skills, and abilities to perform the job (comparable to 4+ years).
In some cases, an equivalency, consisting of a combination of appropriate education, training and/or directly related experience, will be considered sufficient for an individual to meet the requirements of the role.

KNOWLEDGE, SKILLS AND ABILITIES:

• Proficient in Microsoft Excel and Word
• Relevant research and development experience in an industrial, academic, or government laboratory setting
• Hands-on experience with aseptic small scale (plate- to shake flask-based) mammalian cell culture (thawing, expanding, freezing), transfection techniques, and clonal isolation
• Hands-on experience with common nucleic acid- and protein-based in-process analytic assays (e.g., PCR, RTPCR, ELISA, SDS-PAGE, Western blot)
• Experience with mammalian cell culture is preferred
• Fluency in English
• Proven ability to interpret data by performing trend analysis
• Proven ability in technical writing skills
• Ability to independently optimize analytical methods
• Proven problem solving and troubleshooting abilities
• Good written and oral communication skills
• Time management and project management skills
• Ability to work in a collaborative work environment with a team

Task 1: Support lead scientist to deliver top candidates of site-specific integration (SSI) platform in the Client’s Vx CHO cells through a genome-wide screening approach
Deliverables:
a. Design and synthesis (outsourcing) of necessary constructs
b. Production of stable pools and clones
c. Screening for clones with target profile (stability and productivity)
Task 2: Support lead scientist to reverse-engineer pre-existing high-producing CHO clones into PoC SSI candidates
Deliverables:
a. Design and synthesis (outsourcing) of necessary constructs
b. Production of engineered CHO clones
c. Screening for clones with targeted insertion profile