Technician iPSC NIM

at  Springer Nature

TECHNICIAN IPSC NIM

Employer
LCSB – Luxembourg Centre for Systems Biomedicine
Location
Luxembourg
Salary
Negotiable
Closing date
3 Mar 2024
Discipline
Biomedicine, Life Science
Job Type
Bioinformatician, Engineer, Project Manager, Research Assistant, Researcher
Employment - Hours
Full time
Duration
Fixed term
Qualification
Undergraduate
Sector
Academia

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ABOUT US…

The Luxembourg Centre for Systems Biomedicine (LCSB) is an interdisciplinary research centre of the University of Luxembourg. We conduct fundamental and translational research in the field of Systems Biology and Biomedicine - in the lab, in the clinic and in silico. We focus on neurodegenerative processes and are especially interested in Alzheimer’s and Parkinson’s disease and their contributing factors.
The LCSB recruits talented scientists from various disciplines: computer scientists, mathematicians, biologists, chemists, engineers, physicists and clinicians from more than 50 countries currently work at the LCSB. We excel because we are truly interdisciplinary and together we contribute to science and society.
Successful candidates will join the Neuroinflammation group, led by Prof. Michael Heneka, which aims at understanding molecular mechanisms of inflammatory regulation in a variety of neurodegenerative diseases, using novel preclinical mouse models and state-of-the-art techniques like two-photon imaging, transcriptome analysis and induced pluripotent stem cells (iPSCs), to develop new biomarkers and medical intervention programs. For more information, please visit our website

• You will be responsible for the generation of iPSC lines from patient fibroblast or blood samples and for differentiating iPSCs into different cell types (neurons, microglia…)
• You will develop and work with iPSC-derived cell models, prepare primary neuronal and non-neuronal cell cultures, and, in particular, live cell imaging using light and fluorescence microscopy
• You will use sophisticated live cell imaging protocols for the analysis of intra- and intercellular transport
• You will support daily lab operations, including ordering lab supplies, sample organization and preparation as well as biomolecular extraction
• Additional tasks represent methods of cellular and molecular biology including but not restricted to the extraction of biomolecules (DNA, RNA, proteins) and related detection methods (FACS, ELISA, Western blot, qPCR)
  The open position is part of the recently received PEARL grant from the Fonds National de la Recherche in Luxembourg which aims to study the role of microglia neuron interactions in Alzheimer’s disease.
  The project will test if the interaction of immune cells and neurons can be harnessed for developing preventive, disease-modifying or acute therapeutic measures. MINIALZ will further identify early immune function and biomarker traits, in order to support diagnosis prior to clinical appearance. MINIALZ will be based on a new mechanism recently identified by the applicant’s group, by which microglia form a tunnelling nanotube network (TNTs) with neighboring neurons in order to free the latter from pathological proteins and ensure their function and survival. Preliminary data suggest that such microglia-neuron TNTs may contribute to neuronal survival in models of neurodegeneration and the involved mechanisms are defective in microglia carrying disease-associated AD risk mutations. The technician will contribute to the project by generating and maintaining the various cell cultures for the cell culture experiments as well as by performing various biochemical and imaging studies on the iPSCs and differentiated brain cells.
  For further information, please contact Michael Heneka ().